|
Flotation for big animals
|
|
Flotation for small animals
|
|
Sedimentation for big animals
|
|
Sedimentation for small
animals
|
|
.
|
|
Flotation for
the human medicine
|
|
Sedimentation
for the human medicine
|
BIG ANIMALS
Centrifugal flotation
method with ParasiTrap® Flotation PLUS, developed especially
for big animals
|
|
Insert sample just
shortly before starting examination.
1) Insert 2x10 cm³ faecal material onto the sample vessel (use
the additional 10 cm³ shovel).
|
|
|
2) Disperse intensively the sample with the spoon.
|
|
|
3) First filtration of faecal suspension through the one-way
filter.
|
|
|
4) Fill the processing tube I up to the edge (arrow). Insert
approx. 6 ml filtrate.
|
|
|
5) Screw the processing tube II with integrated filter system
onto the processing tube I.
The assembled system is rotated 180º.
|
|
|
6) Mixed by shaker at max. rotary speed
for approx. 10-15 sec. After this operation already half of the stool
suspension will be collected in the lower cone-shaped
processing tube II. If no shaker is available, the system is
shaken thoroughly by hand for approx. 30 sec.
|
|
|
7) The sample in the upper vessel is transferred into the
lower tube II by shaking in vertical direction (active fine
filtration).
|
|
|
8) The filtered faecal suspension is centrifuged for 5 min. at
250-300g (separation of debris).
*Processing with small size
centrifuge and quality control see below.
|
|
|
9) Tube II is filled with ParasiTrap®
Flotation PLUS Medium to
form a slight positive meniscus.
|
|
|
10) Wait 10-20 min. to concentrate
parasites in the upper layer of meniscus (flotation step).
Alternative methods
sampling the reverse meniscus: by cover-slip or glass
slide, take a loop, glass rod or fine pipette.
|
|
|
11) Microscopical examination.
|
|
|
|
SMALL ANIMALS
Centrifugal flotation
method with ParasiTrap® Flotation PLUS, developed especially
for small animals
|
|
Insert sample just
shortly before starting examination.
1) Faecal samples are taken by using the
plunger of processing tube I (auxiliary device spoon).
Don´t overload the plunger (max. full to the brin).
Insert plunger carefully into the
tube I.
|
|
|
2) Close tightly the tube. the closed tube
I should be shaken intensively several times.
|
|
|
3) If necessary, the faecal suspension can
be stirred with the plunger. You lift the screw cap and
insert it quickly again until it touches the brim of the
tube.
|
|
Don´t lift the
plunger above the surface of the fluid!
|
|
4) Remove the screw cap
with the plunger.
|
|
|
5) Screw the processing tube II with integrated filter system
onto the processing tube I.
The assembled system is rotated 180º.
|
|
|
6) Mixed by shaker at max. rotary speed
for approx. 10-15 sec. After this operation already half of the stool
suspension will be collected in the lower cone-shaped
processing tube II. If no shaker is available, the system is
shaken thoroughly by hand for approx. 30 sec.
|
|
|
7) The sample in the upper vessel is transferred into the
lower tube II by shaking in vertical direction (active fine
filtration).
|
|
|
8) The filtered faecal suspension is centrifuged for 5 min. at
250-300g (separation of debris).
*Processing with small size
centrifuge and quality control see below.
|
|
|
9) Tube II is filled with ParasiTrap®
Flotation PLUS Medium to
form a slight positive meniscus.
|
|
|
10) Wait 10-20 min. to concentrate
parasites in the upper layer of meniscus (flotation step).
Alternative methods
sampling the reverse meniscus: by cover-slip or glass
slide, take a loop, glass rod or fine pipette.
|
|
|
11) Microscopical examination.
|
|
|
|
BIG ANIMALS
Sedimentation
with ParasiTrap®
AF/SAF/ECO/Bailenger system for big animals
|
|
1) Insert 2x10 cm³ faecal material onto the sample vessel (use
the additional 10 cm³ shovel).
|
|
|
2) Disperse intensively the sample with the spoon.
|
|
|
3) First filtration of faecal suspension through the one-way
filter.
|
|
|
4) Wait 5-10 min. and take 2,0 ml
out
with the additional big pipette from the bottom. Fill the
content of pipette into the tube I.
|
|
|
5) Add 1,5 ml Combi Medium for separation
and staining.
|
|
|
6) Screw the processing tube II with integrated filter system
onto the processing tube I.
The assembled system is rotated 180º.
|
|
|
7) Mixed by shaker at max. rotary speed
for approx. 10-15 sec. After this operation already half of the stool
suspension will be collected in the lower cone-shaped
processing tube II. If no shaker is available, the system is
shaken thoroughly by hand for approx. 30 sec.
|
|
|
8) The sample in the upper vessel is transferred into the
lower tube II by shaking in vertical direction (active fine
filtration).
|
|
|
9)
The filtered faecal suspension is centrifuged for 5 min. at
1000g.
*Processing with small size
centrifuge and quality control see below.
|
|
|
10) After centrifugation four layers have
been formed in the processing tube II. The tube I including
the filter-piece is removed.
|
|
|
11) The top layer which is solid has to be
detached from tube´s wall by a cotton wool stick.
|
|
|
12) Resuspend the parasites concentrate
according to demand with 0,05-0,5 ml physiological saline
solution or Medium A.
|
|
|
13) For diagnosis usually 1-3 drops of
concentrated stool suspension dilution per slide will be
sufficient. Make sure that your preparation is not too thick.
You must be able to read through your preparation clearly.
|
|
|
14) Microscopical examination.
|
|
|
|
SMALL ANIMALS
Sedimentation
with ParasiTrap®
AF/SAF/ECO/Bailenger system for small
animals
|
|
1) Faecal samples are taken by using the
plunger of processing tube I (auxiliary device spoon).
Don´t overload the plunger (max. full to the brin).
Insert plunger carefully into the
tube I.
|
|
|
2) Close tightly the tube. the closed tube
I should be shaken intensively several times.
|
|
|
3) If necessary, the faecal suspension can
be stirred with the plunger. You lift the screw cap and
insert it quickly again until it touches the brim of the
tube.
|
|
Don´t lift the
plunger above the surface of the fluid!
|
|
4) Remove the screw cap
with the plunger.
|
|
|
5) Add 1,5 ml Combi Medium for separation
and staining.
|
|
|
6) Screw the processing tube II with integrated filter system
onto the processing tube I.
The assembled system is rotated 180º.
|
|
|
7) Mixed by shaker at max. rotary speed
for approx. 10-15 sec. After this operation already half of the stool
suspension will be collected in the lower cone-shaped
processing tube II. If no shaker is available, the system is
shaken thoroughly by hand for approx. 30 sec.
|
|
|
8) The sample in the upper vessel is transferred into the
lower tube II by shaking in vertical direction (active fine
filtration).
|
|
|
9) The filtered faecal suspension is centrifuged for 5 min. at
1000g.
*Processing with small size
centrifuge and quality control see below.
|
|
|
10) After centrifugation four layers have
been formed in the processing tube II. The tube I including
the filter-piece is removed.
|
|
|
11) The top layer which is solid has to be
detached from tube´s wall by a cotton wool stick.
|
|
|
12) Resuspend the parasites concentrate
according to demand with 0,05-0,5 ml physiological saline
solution or Medium A.
|
|
|
13) For diagnosis usually 1-3 drops of
concentrated stool suspension dilution per slide will be
sufficient. Make sure that your preparation is not too thick.
You must be able to read through your preparation clearly.
|
|
|
14) Microscopical examination.
|
|
|
Centrifugal flotation
method with ParasiTrap® Flotation PLUS, developed especially
for the human
medicine
|
|
Insert sample just
shortly before starting examination.
1) Stool samples are taken by using the
plunger of processing tube I (auxiliary device spoon).
Don´t overload the plunger (max. full to the brin).
Insert plunger carefully into the
tube I.
|
|
|
2) Close tightly the tube. the closed tube
I should be shaken intensively several times.
|
|
|
3) If necessary, the stool suspension can
be stirred with the plunger. You lift the screw cap and
insert it quickly again until it touches the brim of the
tube.
|
|
Don´t lift the
plunger above the surface of the fluid!
|
|
4) Remove the screw cap
with the plunger.
|
|
|
5) Screw the processing tube II with integrated filter system
onto the processing tube I.
The assembled system is rotated 180º.
|
|
|
6) Mixed by shaker at max. rotary speed
for approx. 10-15 sec. After this operation already half of the stool
suspension will be collected in the lower cone-shaped
processing tube II. If no shaker is available, the system is
shaken thoroughly by hand for approx. 30 sec.
|
|
|
7) The sample in the upper vessel is transferred into the
lower tube II by shaking in vertical direction (active fine
filtration).
|
|
|
8) The filtered faecal suspension is centrifuged for 5 min. at
250-300g (separation of debris).
*Processing with small size
centrifuge and quality control see below.
|
|
|
9) Tube II is filled with ParasiTrap®
Flotation PLUS Medium to
form a slight positive meniscus.
|
|
|
10) Wait 10-20 min. to concentrate
parasites in the upper layer of meniscus (flotation step).
Alternative methods
sampling the reverse meniscus: by cover-slip or glass
slide, take a loop, glass rod or fine pipette.
|
|
|
11) Microscopical examination.
|
|
|
|
Sedimentation method with
ParasiTrap® AF/SAF/ECO/Bailenger
system for the human medicine
|
|
1) Stool samples are taken by using the
plunger of processing tube I (auxiliary device spoon).
Don´t overload the plunger (max. full to the brin).
Insert plunger carefully into the
tube I.
|
|
|
2) Close tightly the tube. the closed tube
I should be shaken intensively several times.
|
|
|
3) If necessary, the stool suspension can
be stirred with the plunger. You lift the screw cap and
insert it quickly again until it touches the brim of the
tube.
|
|
Don´t lift the
plunger above the surface of the fluid!
|
|
4) Remove the screw cap
with the plunger.
|
|
|
5) Add 1,5 ml Combi Medium for separation
and staining
|
|
|
6) Screw the processing tube II with integrated filter system
onto the processing tube I.
The assembled system is rotated 180º.
|
|
|
7) Mixed by shaker at max. rotary speed
for approx. 10-15 sec. After this operation already half of the stool
suspension will be collected in the lower cone-shaped
processing tube II. If no shaker is available, the system is
shaken thoroughly by hand for approx. 30 sec.
|
|
|
8) The sample in the upper vessel is transferred into the
lower tube II by shaking in vertical direction (active fine
filtration).
|
|
|
9) The filtered faecal suspension is centrifuged for 5 min. at
1000g.
*Processing with small size
centrifuge and quality control see below.
|
|
|
10) After centrifugation four layers have
been formed in the processing tube II. The tube I including
the filter-piece is removed.
|
|
|
11) The top layer which is solid has to be
detached from tube´s wall by a cotton wool stick.
|
|
|
12) Resuspend the parasites concentrate
according to demand with 0,05-0,5 ml physiological saline
solution or Medium A.
|
|
|
13) For diagnosis usually 1-3 drops of
concentrated stool suspension dilution per slide will be
sufficient. Make sure that your preparation is not too thick.
You must be able to read through your preparation clearly.
|
|
|
14) Microscopical examination.
|
|
|
|
*Processing with small size
centrifuge and quality control.
Please remove the tube I
with the filter and screw the additional screw cap on the
tube II.
|
|
|
|
